Technologies and Engineering

Development of method of estimation efficiency of decontamination of phosphororganic compounds

Purpose. Development of a method for evaluating the efficiency of decontamination in terms of the integral effects of the splitting of toxic substances on the human body. Methodology. Paraoxon is the model substance of the characteristic chemical structure and toxicological characteristics. As a model system for determining the toxicity of paraoxone and its alkaline hydrolysis products, the inhibition of human serum butyrylcholinesterase was used. Determination of butyrylcholinesterase activity was performed ex vivo spectrophotometrically using the modified Ellman method. Findings. It has been found that products of alkaline hydrolysis of paraoxone are almost 85 times less effective butyrylcholinesterase inhibitors of human serum than paraoxon. It was shown that the analysis of kinetics of inhibition of human blood serum butyrylcholinesterase in ex vivo conditions can be considered a promising quantitative method for assessing the effectiveness of the decontamination method of toxic organophosphorus compounds from the point of view of the effects on the human organism of cleavage products. Originality. For the first time, the method of complex quantitative assessment of the efficiency of decontamination systems of organophosphorus compaunds from the point of view of the integral effects of the splitting of toxic substances on the human body has been proposed. Practical value. Based on the evaluation of the effect of phosphorus organic compounds on the inhibition of butyrylcholinesterase in human blood serum, the most effective method for chemical decontamination of toxic substances can be selected, objectively evaluated approaches and degassing systems at the development stage, methods for correcting the composition of detergent decontamination agents for technological equipment in chemical and pharmaceutical industries

decontamination, organophosphorus compounds, efficacy, evaluation method, butyrylcholinesterase, toxic substances